Document Type : Original Article

Author

• Nuha Hussam Abdulwahab

جامعة تكريت

Abstract

Methotrexate (MTX) is an analog of folic acid utilized as a low-dose treatment for autoimmune diseases, such as PsA or RA and a high-dose treatment for cancer. MTX increased the apoptosis by switching on caspase 3, which is switching on death protease, stimulating the specific incision of cellular proteinase. We chose BSE for this research because it content from glucoraphanin higher than adult broccoli, This research aimed to determine the levels of casp -3 and liver function to determinated protective effects of BSE in mitigating MTX impact in rat female due to lack of studies that addressed this matter especially in females which spread among them lots of autoimmune diseases requires MTX use. RT-qPCR technique was used to determination levels of gene expression subsequent to RNA extraction. Our study suggested that BSE enhance liver functions and caspase 3 expression in rat that treated with MTX and broccoli sprout extract and in groups treated with BSE only if we compared it with control and MTX groups.

Keywords

• Methotrexate(MTX)
• Broccli sproute extract (BSE)
• cysteine-containing aspartic acid-specific proteases) CASP3 Alanine aminotranseferase(ALT)
• Aspartate aminotraneferase(AST)
• Alkaline phosphatase (ALP