Document Type : Original Article
Author
Abstract
This study aimed to assess the relationship between sperm DNA fragmentation and semen quality indicators
in infertile males. 30 semen samples from fertile controls and 60 from infertile males with aberrant semen
parameters made up the 90 semen samples examined. The semen was analyzed using the WHO (2010)
standards, which include leukocyte count, morphology, motility, and sperm concentration. DNA damage was
evaluated using the aniline blue staining method, and samples were categorized based on the extent of
fragmentation.
All analyzed factors show statistically significant differences (P<0.001), according to the results. The normal
group's mean sperm concentration was significantly greater at 54.93±13.44 million/ml than the abnormal
group's, which was 17.10±15.24 million/ml. Similarly, the aberrant semen group's progressive motility was
significantly lower (19.53±15.30%) than that of the normal group (56.43±16.85%). The normal group's sperm
morphology (61.67±13.08%) was superior to that of the abnormal group (36.33±14.25%). Additionally, the
normal group's semen volume (3.85±1.18 ml) was substantially greater than that of the abnormal group
(3.26±1.47 ml). Furthermore, the abnormal semen group's leukocyte concentration (1.92±1.21 million/ml)
was substantially higher than that of the normal group (0.68±0.28 million/ml).
According to the results, abnormal samples had significantly more DNA damage (49.50 ± 27.50) than normal
samples (19.10 ± 5.31). Sperm DNA damage was much higher in the abnormal group than in the control group
(P < 0.001). There was a statistically significant negative correlation between DNA fragmentation and sperm
motility (r = -0.47, P = 0.001), morphology (r = -0.44, P = 0.001), and concentration (r = -0.41, P = 0.001).
There was also a somewhat positive correlation between DNA fragmentation and leukocytes (r = 0.21, P =
0.052). This study found that infertile guys' low semen quality is closely correlated with greater sperm DNA
fragmentation, which might be a good indicator for male infertility diagnosis.
Keywords
)