Document Type : Original Article
Author
Abstract
The analysis of DNA sequences plays a critical role in genetic research, but challenges persist in attaining
high precision with mixed sequences. This study aims to evaluate the efficiency of the Mixed Sequence
Analysis application in analyzing DNA sequences with heterozygous base calling as well as manual analysis.
DNA was extracted from 31 unrelated Iraqi individuals, and D13S317 is one specific STR locus that is
highly variable among individuals and was amplified. The mixed sequence was then analyzed using the
Mixed Sequence Analysis tool, complemented by manual chromatogram interpretation. The most frequent
allele detected was TATC 12 (52.27%), followed by TATC 9 (20.45%). The least frequent allele was
TATC10, which was 4.55%; also, different alleles were detected with high variability. The analysis revealed
that while the application was beneficial, there were challenges in distinctive variations at the base level due
to noise in the chromatogram. Despite its limitations, this research highlights the importance of developing
accurate sequencing analysis techniques, particularly in situations where other methods are unavailable.
Keywords
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