Document Type : Original Article
Author
Abstract
Objective: Isolation the bacteria from food source has a probiotic characterization and evaluate the
inhibitory activity against bacterial pathogens. Methods: Lactic acid bacteria from various sources
are isolated by the MRS-CaCO3Agar medium, the isolates were purified and microscopic,
biochemical were tested. The antibacterial activity of cell free supernatant (CFS) against Bacillus
subtilis , Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli also testing. The
isolate was selected based on high inhibitory activity and genetic diagnosis were performed. Results:
the highest inhibitory diameter against Gram-positive bacteria was recorded with a diameter of 13
mm to Staph aureus. The results showed that the bacterial isolate Gram positive, immobile, negative
for catalyze, Esculin hydrolysis, and CO2 production from glucose, grows at 10- 45⸰C, 2- 6.5% Nacl,
and at pH 4.5- 6.9. It had the ability to ferment of sucrose, mannitol, arabinose, sorbitol and xylose.
The diagnosis of isolation has been genetically confirmed to the species Enterococcus faecium.
Conclusion: The E. faecium can be considered as probiotics in a safe manner to adaptive immune, in
addition to the possibility of using it as a potential source of natural antibacterial agent in food and
pharmaceutical industries.
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