Document Type : Original Article
Author
Abstract
This study involved two plant species of different plant families, cumin (Cuminum
cyminum) family Apiaceae and basil (Ocimum basilicum) family Lamiaceae of medical
and economic importance. Aim: study luteolin o -7- glucoside in both species and evaluate
its potential effect on colon cancer.
Method: extraction by (70% ethanol) using (Soxhlet and reflux), high-performance liquid
chromatography (HPLC) for detection of compound , and in vitro evaluation of the
potential biological activity of crude plant extracts and luteolin O-7 glycoside with
anticancer activity of Colon HCT Cell lines were tested using (MTT) assay.
Results: of in vitro anticancer activity study conducted against colon cancer cell line
showed moderate cytotoxicity of these plant extracts and luteolin 0 7 glucoside
compounds against colon cancer. In which inhibition in viability of colon cancer cell by
Cuminum cyminum (17.48% at concentration 250µg , 18.39 at concentration 500µg and 34
at 1000µg ) , Ocimum basilicum showed inhibition in the viability of cancer cell (17.66 at
250 µg , 26.8 500µg and 40.6 at 1000µg) While luteolin 0 - 7-glucoside stander showed
decrease in the viability of cancer cell by (29.14 at 250 µg 11.11 500µg and 39 at 1000µg
Keywords
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