Document Type : Original Article
Author
Abstract
Diarrhea is a significant cause of illness and death in many parts of the world
and people of all ages. Fecal-oral transmission and intake of food and water polluted
with pathogenic organisms are the major causes of acute infectious diarrhea, based on
research. This investigation was carried out to isolate various bacteria that cause
diarrhea in children below 5 years, and 80 stool samples were collected The study
included the effect of age and area of residence on the incidence of diarrhea. These
samples were planted on selective and differential culture media, which included the
media of the MacConkey agar and blood agar, as well as the use of biochemical tests
to diagnose these isolates using Vitek compact 2. The results of the study showed that
60 bacteria isolates belonging to seven different genera were obtained, Escherichia
coli, SalmonellaTyphimurium, Klebsiella pneumonia, Shigella spp, Pseudomonas
aeruginosa, Enterobacter aeruginosa, and Proteus mirabilis. And at the rates of
23.33%, 20%, 16.66%, 16.66%, 15%, 5%, and 3.33%, respectively. The results
showed that the age group (1-2 years) recorded a high percentage of diarrhea. The
sensitivity test showed that all the isolates were 100% sensitive to Ciprofloxacin and
100% Amoxicillin resistant, while these isolates varied in their sensitivity to the rest
of the antibiotics. Finally, this investigation confirmed that diarrhea in children under
the age of five was caused by multiple-drug resistant, gram-negative enterobacteria.
Keywords
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