Document Type : Original Article
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Abstract
Infertility is the most common endocrine disease occurring in women of reproductive age. The aim of study to determine the D2 dopamine receptors gene expression and clarify altered concentrations of dopamine in infertile women and controls. Also, to assess their role in infertility and their correlation with measured biochemical parameters.This study was carried out in the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies - University of Baghdad through the period from January 2023 - April 2023, Methods, The patients were taken from the Kamal Al-Samarraee Infertility Treatment Hospital in Baghdad; the study was included 100 individuals 50 controls and 50 infertile females diagnosed with polycystic ovary syndrome (PCOS ). Biochemical examinations were done include Serum concentrations of Dopamine (DA), luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin were measured by Enzyme-linked Immuno sorbent assay ELISA and Automated Immune Assay (AIA) kits. The RNA was extracted from whole fresh blood. The Concentration and Purity of RNA Based on a nanodrop device were performed, then converted to cDNA to measure the level of gene expression for DRD2 by using Real-Time PCR technique, then converted to cDNA to measure the level of gene expression for DRD2 by using Real-Time PCR technique. Results: In this study, the results showed a significant decrease in Dopamine levels in infertile Women in comparison to the control group (P< 0.01). In addition, the analysis of hormone levels (LH), (FSH) and (PRL) in infertile females showed a highly significant increase in PRL and LH compared to the control group (P≤0.01) and a non-significant difference in the level of (FSH). The results of the fold expression of DRD2 showed down-regulated in patients, whereas it was up-regulated in the control group. In conclusion, According to the results of gene expression of the DRD2 gene, Dopamine can be utilized as a biomarker for early diagnosis of female infertility in Iraqi females as one of the laboratory diagnostic methods.
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